By default you can uploadasp.netapplication to wwwroot folder and run website. If you want to hostasp.netapplication in a subfolder, you have to configure virtual directory for the sub-folder. Please follow instruction below to create virtual directory in DotNetPanel 1. Login DotNetPanel 2. Navigate to hosting space -> Web Sites 3. Select website you want to hostasp.netapplication -> Virtual Dirs You can create a Virtual Directory with the same name as actual directory.